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prk5-ulk1-myc  (Addgene inc)


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    Structured Review

    Addgene inc prk5-ulk1-myc
    Prk5 Ulk1 Myc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+ulk1+myc/prk5+ha+ubiquitin+wt/pmc10529509-282-18-31
    Average 90 stars, based on 1 article reviews
    prk5-ulk1-myc - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Activation of autophagy depends on Atg1/Ulk1-mediated phosphorylation and inhibition of the Hsp90 chaperone machinery
    Article Snippet: .. Hsp82-His 6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously., pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR, constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter ( ADH1 ), the GRE reporter vector pΔS26X, a URA3 vector which expresses β-galactosidase (encoded by lacz ) as a reporter gene under control of a promoter bearing 3×GR response elements was reported previously. ..

    Article Title: Activation of autophagy depends on Atg1/Ulk1-mediated phosphorylation and inhibition of the Hsp90 chaperone machinery.
    Article Snippet: Hsp82-His6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously.37,38 pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR,75 constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter (ADH1), theGRE reporter vector pDS26X, aURA3 vector which expresses b-galactosidase (encoded by lacz) as a reporter gene under control of a promoter bearing 33GR response elements76 was reported previously.80 V5-ERa and V5-AR constitutively expressing estrogen receptor-alpha (ERa) and androgen receptor (AR), respectively, under control of the Glycerol-3-Phosphate Dehydrogenase (GPD1) promoter were previously described.77 pUCdeltaSS-ERE,78 2xRLM1-LacZ reporter plasmid,47 4XHSE-LacZ-pUp41a,47 YpRS426GAL1-v-Src plasmid,79 STE11DN-myc-pYES2,80 and pcDNA3-Cdc37-FLAG WT81 were all reported previously. .. Hsp82-His6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously.37,38 pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR,75 constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter (ADH1), theGRE reporter vector pDS26X, aURA3 vector which expresses b-galactosidase (encoded by lacz) as a reporter gene under control of a promoter bearing 33GR response elements76 was reported previously.80 V5-ERa and V5-AR constitutively expressing estrogen receptor-alpha (ERa) and androgen receptor (AR), respectively, under control of the Glycerol-3-Phosphate Dehydrogenase (GPD1) promoter were previously described.77 pUCdeltaSS-ERE,78 2xRLM1-LacZ reporter plasmid,47 4XHSE-LacZ-pUp41a,47 YpRS426GAL1-v-Src plasmid,79 STE11DN-myc-pYES2,80 and pcDNA3-Cdc37-FLAG WT81 were all reported previously. ..

    Expressing:

    Article Title: Activation of autophagy depends on Atg1/Ulk1-mediated phosphorylation and inhibition of the Hsp90 chaperone machinery
    Article Snippet: .. Hsp82-His 6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously., pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR, constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter ( ADH1 ), the GRE reporter vector pΔS26X, a URA3 vector which expresses β-galactosidase (encoded by lacz ) as a reporter gene under control of a promoter bearing 3×GR response elements was reported previously. ..

    Article Title: Activation of autophagy depends on Atg1/Ulk1-mediated phosphorylation and inhibition of the Hsp90 chaperone machinery.
    Article Snippet: Hsp82-His6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously.37,38 pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR,75 constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter (ADH1), theGRE reporter vector pDS26X, aURA3 vector which expresses b-galactosidase (encoded by lacz) as a reporter gene under control of a promoter bearing 33GR response elements76 was reported previously.80 V5-ERa and V5-AR constitutively expressing estrogen receptor-alpha (ERa) and androgen receptor (AR), respectively, under control of the Glycerol-3-Phosphate Dehydrogenase (GPD1) promoter were previously described.77 pUCdeltaSS-ERE,78 2xRLM1-LacZ reporter plasmid,47 4XHSE-LacZ-pUp41a,47 YpRS426GAL1-v-Src plasmid,79 STE11DN-myc-pYES2,80 and pcDNA3-Cdc37-FLAG WT81 were all reported previously. .. Hsp82-His6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously.37,38 pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR,75 constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter (ADH1), theGRE reporter vector pDS26X, aURA3 vector which expresses b-galactosidase (encoded by lacz) as a reporter gene under control of a promoter bearing 33GR response elements76 was reported previously.80 V5-ERa and V5-AR constitutively expressing estrogen receptor-alpha (ERa) and androgen receptor (AR), respectively, under control of the Glycerol-3-Phosphate Dehydrogenase (GPD1) promoter were previously described.77 pUCdeltaSS-ERE,78 2xRLM1-LacZ reporter plasmid,47 4XHSE-LacZ-pUp41a,47 YpRS426GAL1-v-Src plasmid,79 STE11DN-myc-pYES2,80 and pcDNA3-Cdc37-FLAG WT81 were all reported previously. ..

    Control:

    Article Title: Activation of autophagy depends on Atg1/Ulk1-mediated phosphorylation and inhibition of the Hsp90 chaperone machinery
    Article Snippet: .. Hsp82-His 6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously., pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR, constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter ( ADH1 ), the GRE reporter vector pΔS26X, a URA3 vector which expresses β-galactosidase (encoded by lacz ) as a reporter gene under control of a promoter bearing 3×GR response elements was reported previously. ..

    Article Title: Activation of autophagy depends on Atg1/Ulk1-mediated phosphorylation and inhibition of the Hsp90 chaperone machinery.
    Article Snippet: Hsp82-His6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously.37,38 pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR,75 constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter (ADH1), theGRE reporter vector pDS26X, aURA3 vector which expresses b-galactosidase (encoded by lacz) as a reporter gene under control of a promoter bearing 33GR response elements76 was reported previously.80 V5-ERa and V5-AR constitutively expressing estrogen receptor-alpha (ERa) and androgen receptor (AR), respectively, under control of the Glycerol-3-Phosphate Dehydrogenase (GPD1) promoter were previously described.77 pUCdeltaSS-ERE,78 2xRLM1-LacZ reporter plasmid,47 4XHSE-LacZ-pUp41a,47 YpRS426GAL1-v-Src plasmid,79 STE11DN-myc-pYES2,80 and pcDNA3-Cdc37-FLAG WT81 were all reported previously. .. Hsp82-His6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously.37,38 pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR,75 constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter (ADH1), theGRE reporter vector pDS26X, aURA3 vector which expresses b-galactosidase (encoded by lacz) as a reporter gene under control of a promoter bearing 33GR response elements76 was reported previously.80 V5-ERa and V5-AR constitutively expressing estrogen receptor-alpha (ERa) and androgen receptor (AR), respectively, under control of the Glycerol-3-Phosphate Dehydrogenase (GPD1) promoter were previously described.77 pUCdeltaSS-ERE,78 2xRLM1-LacZ reporter plasmid,47 4XHSE-LacZ-pUp41a,47 YpRS426GAL1-v-Src plasmid,79 STE11DN-myc-pYES2,80 and pcDNA3-Cdc37-FLAG WT81 were all reported previously. ..

    Plasmid Preparation:

    Article Title: Activation of autophagy depends on Atg1/Ulk1-mediated phosphorylation and inhibition of the Hsp90 chaperone machinery
    Article Snippet: .. Hsp82-His 6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously., pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR, constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter ( ADH1 ), the GRE reporter vector pΔS26X, a URA3 vector which expresses β-galactosidase (encoded by lacz ) as a reporter gene under control of a promoter bearing 3×GR response elements was reported previously. ..

    Article Title: Activation of autophagy depends on Atg1/Ulk1-mediated phosphorylation and inhibition of the Hsp90 chaperone machinery.
    Article Snippet: Hsp82-His6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously.37,38 pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR,75 constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter (ADH1), theGRE reporter vector pDS26X, aURA3 vector which expresses b-galactosidase (encoded by lacz) as a reporter gene under control of a promoter bearing 33GR response elements76 was reported previously.80 V5-ERa and V5-AR constitutively expressing estrogen receptor-alpha (ERa) and androgen receptor (AR), respectively, under control of the Glycerol-3-Phosphate Dehydrogenase (GPD1) promoter were previously described.77 pUCdeltaSS-ERE,78 2xRLM1-LacZ reporter plasmid,47 4XHSE-LacZ-pUp41a,47 YpRS426GAL1-v-Src plasmid,79 STE11DN-myc-pYES2,80 and pcDNA3-Cdc37-FLAG WT81 were all reported previously. .. Hsp82-His6 with a PreScission protease cleavage site introduced between the amino-domain and charged linker region was cloned previously.37,38 pRK5-Ulk1-myc (#31961), GFP-Atg8 (#49425), pJK59-GFP-Atg17 (#44175) and ptfLC3 (#21074) were purchased from Addgene. pHCA/rGR,75 constitutively expressing glucocoticoid receptor (GR) under control of the Alcohol dehydrogenase promoter (ADH1), theGRE reporter vector pDS26X, aURA3 vector which expresses b-galactosidase (encoded by lacz) as a reporter gene under control of a promoter bearing 33GR response elements76 was reported previously.80 V5-ERa and V5-AR constitutively expressing estrogen receptor-alpha (ERa) and androgen receptor (AR), respectively, under control of the Glycerol-3-Phosphate Dehydrogenase (GPD1) promoter were previously described.77 pUCdeltaSS-ERE,78 2xRLM1-LacZ reporter plasmid,47 4XHSE-LacZ-pUp41a,47 YpRS426GAL1-v-Src plasmid,79 STE11DN-myc-pYES2,80 and pcDNA3-Cdc37-FLAG WT81 were all reported previously. ..



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